Regulatory

Part:BBa_K112322:Design

Designed by: Aron Lau   Group: iGEM08_UC_Berkeley   (2008-10-22)


{Pdps} in BBb format


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 287
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR JH089F/JH089R on Salmonella L2.    (379 bp, EcoRI/BamHI/DpnI)
Sub into pBjh1601CK             (EcoRI/BamHI, 3134+910, L)
Product is pBjh1601CK-K112322
-----------------------------------------------
JH089F   Forward biobricking of {Pdps}   
cgataGAATTCatgAGATCTaacgattcgacgcgaaccgc
JH089R   Reverse biobricking of {Pdps}   
cgttaGGATCCaatctcatatcctcttgatg


Source

Salmonella L2

References